March 13th, 14th and 17th
On-site event
Medisiina D, C209 and C235
Flyer 

Turku Bioscience Genome Editing Core is organizing a workshop on generating knock-out and knockin modification using CRISPR/Cas9 technology with in vitro ribonucleoprotein complex delivery. During the course, participants will learn how to create specific genetic modifications with the CRISPR/Cas9 system, aspects of designing gRNAs and homology-directed repair templates, and they will gain handson experience by working with 293T cell lines.

Content:

• Introduction to CRISPR/Cas9 system
• gRNA and homology-directed repair template design
• in vitro ribonucleoprotein delivery
• Experimental efficiency analysis

Program and timetable:
13.3. 9:00 – 11:00 Theoretical background, assignation of working groups
14.3. 9:00 – 12:00 RNP assembly and nucleofection
17.3. 9:00 – 12:00 Cell harvest and flow cytometry, data analysis

Participants:
Master’s and PhD students, post-doctoral researchers, researchers
Maximum number of participants: 2

Price:
100 E

Registration:
13 – 28.2.2023
https://konsta.utu.fi/Default.aspx?tabid=88&tap=14693

Further information:
gene_editing@biocience.fi
https://bioscience.fi/events/crispr-workshop/