Lifespan seminar: Studies of post-translational modifications by mass spectrometry reveal histone proteoform dynamics


October 9, 2018    
12:15 pm - 1:15 pm


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Lifespan Research Program Seminar

Prof. Ole N. Jensen, Dept. Biochemistry & Molecular Biology, University of Southern Denmark, Odense, DK
Studies of post-translational modifications by mass spectrometry reveal histone proteoform dynamics

Host: Riitta Lahesmaa ( )

Coffee and cookies served at 12:00

Prof. Ole N. Jensen obtained the degree of PhD in Biochemistry and Biophysics in 1994 at Oregon State University at Corvallis, USA, based on studies of protein-nucleic acid interactions with professor Douglas F. Barofsky. He then joined the research group of Matthias Mann at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany, where he contributed to the development of mass spectrometry tools for the emerging research field of proteomics.

In 1997 he returned to Odense and the University of Southern Denmark as an assistant professor and he initiated studies of protein phosphorylation using affinity enrichment methods and tandem mass spectrometry. He became an associate professor in 2000 and then a Lundbeck Foundation Research Professor 2004-2009. He was awarded an EliteForsk prize from the Danish Ministry of Science in 2009 for his contributions to the development of the field of phosphoproteomics to study cell signaling and regulatory networks.

In recent years, Ole N. Jensen’s research focus has moved to chromatin biology and epigenetics and “middle-down” proteomics approaches and mass spectrometry to study co-existing post-translational modifications and their cross-talk in proteins. Ole N. Jensen is director of the VILLUM Center for Bioanalytical Sciences at SDU, which was inaugurated in 2014 based on a DKK 60M grant from the VILLUM Foundation.  He is also directing PRO-MS, the national Danish research infrastructure for mass spectrometry and functional proteomics.

Selected publications
Accumulation of histone variant H3.3 with age is associated with profound changes in the histone methylation landscape.

Tvardovskiy A, Schwämmle V, Kempf SJ, Rogowska-Wrzesinska A, Jensen ON. Nucleic Acids Res. 2017 Sep 19;45(16):9272-9289. doi: 10.1093/nar/gkx696. PMID: 28934504

Maximizing Sequence Coverage in Top-Down Proteomics By Automated Multi-modal Gas-phase Protein Fragmentation. Shliaha PV, Gibb S, Gorshkov V, Jespersen MS, Andersen GR, Bailey D, Schwartz J, Eliuk S, Schwämmle V, Jensen ON. Anal Chem. 2018 Sep 25. doi: 10.1021/acs.analchem.8b02344. [Epub ahead of print] PMID: 30252444

Systems Level Analysis of Histone H3 Post-translational Modifications (PTMs) Reveals Features of PTM Crosstalk in Chromatin Regulation. Schwämmle V, Sidoli S, Ruminowicz C, Wu X, Lee CF, Helin K, Jensen ON. Mol Cell Proteomics. 2016 Aug;15(8):2715-29. doi: 10.1074/mcp.M115.054460. Epub 2016 Jun 14. PMID: 27302890