CRISPR Workshop 2023


March 13, 2023 - March 17, 2023    
9:00 am - 12:00 pm


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March 13th, 14th and 17th
On-site event
Medisiina D, C209 and C235

Turku Bioscience Genome Editing Core is organizing a workshop on generating knock-out and knockin modification using CRISPR/Cas9 technology with in vitro ribonucleoprotein complex delivery. During the course, participants will learn how to create specific genetic modifications with the CRISPR/Cas9 system, aspects of designing gRNAs and homology-directed repair templates, and they will gain handson experience by working with 293T cell lines.


  • Introduction to CRISPR/Cas9 system
  • gRNA and homology-directed repair template design
  • in vitro ribonucleoprotein delivery
  • Experimental efficiency analysis

Program and timetable:
13.3. 9:00 – 11:00 Theoretical background, assignation of working groups
14.3. 9:00 – 12:00 RNP assembly and nucleofection
17.3. 9:00 – 12:00 Cell harvest and flow cytometry, data analysis

Master’s and PhD students, post-doctoral researchers, researchers
Maximum number of participants: 2

100 E

13 – 28.2.2023

Further information: