BEGIN:VCALENDAR VERSION:2.0 PRODID:-//wp-events-plugin.com//7.3.3//EN BEGIN:VEVENT UID:410@biocityturku.fi DTSTART;TZID=Europe/Helsinki:20201105T180000 DTEND;TZID=Europe/Helsinki:20201105T191500 DTSTAMP:20231013T062914Z URL:https://biocityturku.fi/events/frontiers-of-science-reprogramming-t-ce lls-with-crispr/ SUMMARY:Frontiers of Science: Reprogramming T Cells with CRISPR DESCRIPTION:Associate Prof. Alexander Marson\, University of California\, S an Francisco\, US\n\nhost: Riitta Lahesmaa (riitta.lahesmaa@utu.fi)\n\n&nb sp\;\n\nThe goal of Marson's lab is to understand genetic circuits that co ntrol human immune cell function in health and disease. They employ genomi c techniques to map epigenetic circuits that establish highly specialized cell identities. Marson's team have begun to identify autoimmunity risk va riants that disrupt immune cell circuits\, and how pathogenic circuits may be targeted with novel therapeutics. His lab has developed new tools for CRISPR genome engineering in primary human T cells and they are now pursui ng a comprehensive strategy to test how coding and non-coding genetic vari ation controls functional programs in the immune system. Genome engineered human T cells hold great potential for the next generation of cell-based therapies for cancer\, autoimmunity\, and infectious diseases.\n\nSelected publications\n\nRoth TL\, Li PJ\, Blaeschke F\, Nies JF\, Apathy R\, Mowe ry C\, Yu R\, Nguyen MLT\, Lee Y\, Truong A\, Hiatt J\, Wu D\, Nguyen DN\, Goodman D\, Bluestone JA\, Ye CJ\, Roybal K\, Shifrut E\, Marson A. 2020. Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapi es. Cell. 181: 728-744.e21.\nhttps://dx.doi.org/10.1016/j.cell.2020.03.039 \n\nNguyen DN\, Roth TL\, Li PJ\, Chen PA\, Apathy R\, Mamedov MR\, Vo LT\ , Tobin VR\, Goodman D\, Shifrut E\, Bluestone JA\, Puck JM\, Szoka FC\, M arson A. 2020. Polymer-stabilized Cas9 nanoparticles and modified repair t emplates increase genome editing efficiency. Nat Biotechnol. 38: 44-49\nht tps://dx.doi.org/10.1038/s41587-019-0325-6\n\nShifrut E\, Carnevale J\, To bin V\, Roth TL\, Woo JM\, Bui CT\, Li PJ\, Diolaiti ME\, Ashworth A\, Mar son A. 2018. Genome-wide CRISPR Screens in Primary Human T Cells Reveal Ke y Regulators of Immune Function. Cell. 175: 1958-1971.e15\nhttps://dx.doi. org/10.1016/j.cell.2018.10.024\n\nRoth TL\, Puig-Saus C\, Yu R\, Shifrut E \, Carnevale J\, Li PJ\, Hiatt J\, Saco J\, Krystofinski P\, Li H\, Tobin V\, Nguyen DN\, Lee MR\, Putnam AL\, Ferris AL\, Chen JW\, Schickel JN\, P ellerin L\, Carmody D\, Alkorta-Aranburu G\, Del Gaudio D\, Matsumoto H\, Morell M\, Mao Y\, Cho M\, Quadros RM\, Gurumurthy CB\, Smith B\, Haugwitz M\, Hughes SH\, Weissman JS\, Schumann K\, Esensten JH\, May AP\, Ashwort h A\, Kupfer GM\, Greeley SAW\, Bacchetta R\, Meffre E\, Roncarolo MG\, Ro mberg N\, Herold KC\, Ribas A\, Leonetti MD\, Marson A. 2018. Reprogrammin g human T cell function and specificity with non-viral genome targeting. N ature 559: 405-409\nhttps://dx.doi.org/10.1038/s41586-018-0326-5\n\nSchuma nn K\, Lin S\, Boyer E\, Simeonov DR\, Subramaniam M\, Gate RE\, Haliburto n GE\, Ye CJ\, Bluestone JA\, Doudna JA\, Marson A. 2015. Generation of kn ock-in primary human T cells using Cas9 ribonucleoproteins. Proc Natl Acad Sci U S A 112: 10437-42\nhttps://dx.doi.org/10.1073/pnas.1512503112\n\n&n bsp\;\n\nGenral info regarding the Frontiers of Science eTALKs\n\n Regist ration is obligatory and links to register to all Frontiers of Science Aut umn 2020 seminars are available in BioCity Turku webpage (https://biocityt urku.fi/biocityevents/frontiers-of-science-seminars/programme-autumn-2020/ ) and in our news feed.\n Registration closes two days before the event a nd link to join zoom will be sent to the participants one day in advance.\ n Microphones and cameras need to be turned off during the presentation\, but the audience is welcome to ask questions after the talk\, preferably with video turned on to make the discussion as interactive as possible.\n If you are a student and need a certificate for your attendance\, please fill in your student number when registering to the events. After the semi nar season is over\, please contact BioCity Turku coordinator biocityturku @bioscience.fi.\n\nIf you have any questions regarding our Frontiers of Sc ience eTALK seminar series\, please don’t hesitate to contact BioCity Tu rku coordinator biocityturku@bioscience.fi CATEGORIES:Frontiers of Science END:VEVENT BEGIN:VTIMEZONE TZID:Europe/Helsinki X-LIC-LOCATION:Europe/Helsinki BEGIN:STANDARD DTSTART:20201025T030000 TZOFFSETFROM:+0300 TZOFFSETTO:+0200 TZNAME:EET END:STANDARD END:VTIMEZONE END:VCALENDAR